HPLC is described for determination of the % drug release and estimation of drug in capsule. The method employs a prinstone C8 column (250 x 4.6 mm, 5.0 μ) with an isocratic mixture of Acetonitrile:Water:TEA (600:400:1 v/v/v) pH- 4 adjusted with ortho-phosphoric acid at a flow rate of 1 ml/min and detection at 280 nm. Retention time of Nimesulide and Diclofenac Sodium was 7.6 min and 9.6 min respectively. Method shows linearity in the range of 20 μg/ml - 100 μg/ml for Nimesulide and 10 μg/ml - 50 μg/ml for Diclofenac Sodium with correlation coefficient 0.9993 and 0.9994 for Nimesulide & Diclofenac Sodium respectively. LOD was found to be 0.12 μg/mL and LOQ was found to be 0.37 μg/mL for Diclofenac Sodium and LOD was found to be 1.2 μg/mL and LOQ was found to be 3.6 μg/mL for Nimesulide.
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